トップページ研究室紹介(基礎医学領域)微生物・免疫学 分子病原細菌学/耐性菌制御学(細菌学)

微生物・免疫学

分子病原細菌学/耐性菌制御学(細菌学)

研究室概要

 医療関連感染症および市中感染症で問題となる、ブドウ球菌(MRSAを含む)属、レンサ球菌属、バチルス属菌、大腸菌や肺炎桿菌などの腸内細菌科菌種、緑膿菌やアシネトバクター属菌などのブドウ糖非発酵菌群、ヘモフィルス属菌などについて、病原因子、薬剤耐性機構などの研究を行っています。研究では基本的な細菌学的解析手法に加え、遺伝子解析、LC-MS/MS等を用いたタンパク質解析(プロテオーム解析)などを活用し、細菌感染症の診断/予防/治療に貢献する成果を目指しています。

研究プロジェクト

(1) 病原細菌における薬剤耐性機構、分子疫学解析

(2) 細菌の病原性に関与する諸因子とその発現/産生調節機構の解析

(3) 細菌感染症の制御への応用を目指したゲノム、プロテオーム、メタボローム解析

教員

構成員名/英名表記 役職 所属
荒川 宜親/ARAKAWA Yoshichika
詳しくはこちら→
教授分子病原細菌学/耐性菌制御学
木村 幸司/KIMURA Kouji
詳しくはこちら→
准教授分子病原細菌学/耐性菌制御学
和知野 純一/WACHINO Jun-ichi
詳しくはこちら→
講師分子病原細菌学/耐性菌制御学
金 万春 / Wanchun JIN
助教分子病原細菌学/耐性菌制御学
Anupriya KUMAR
助教分子病原細菌学/耐性菌制御学

研究分野紹介

専攻 分子総合医学専攻
大講座 微生物・免疫学
分野 分子病原細菌学/耐性菌制御学
ユニット名 細菌学

研究キーワード

細菌感染症、病原細菌、薬剤耐性菌

連絡先

Tel

内線: 2106

外線: 052-744-2106

Fax

内線: 2107

外線: 052-744-2107

大学院入学案内 

感染症の発症機序や細菌の耐性機構に興味をお持ちで意欲のある方の応募をお待ちしています。大学院生の卒業学部は問いませんが、医学系、薬学系、農学部など生物系の出身学生が多くいます。卒業生の進路は病院勤務(医師・薬剤師・看護士・臨床検査技師)をはじめ、国家/地方公務員、企業、アカデミックポジションなど。
 また、基礎研究に興味のある学部在籍者も受け入れており、基礎医学セミナーの延長として研究をすることで、国内外の学会発表などを経験しています。感染症は基礎の中でも臨床に直結する学問分野であり、ここで得られる知識や技術は将来大いに役立つと考えます。

近年の研究業績

2016

H. Ohno, J. Wachino, R. Saito, W. Jin, K. Yamada, K. Kimura, Y. Arakawa.

A highly macrolide-resistant Campylobacter jejuni strain with rare A2074T mutations in 23S rRNA genes.
Antimicrobial Agents and Chemotherapy.in press.

Nakane K, Kawamura K, Goto K, Arakawa Y.

Long-term colonization of blaCTX-M-harboring Escherichia coli found in ordinary Japanese people engaged in food handling in community.Appl.Environ.Microbiol.in press.

Fukigai S¶, Morimoto M¶, Kimura K, Doyama Y, Miyazaki A, Kamiya C, Banno H, Morishima E, Onoda T, Nagano N, Jin W, Wachino J, Yamada K, Arakawa Y.

Effectual detection of group B streptococci with reduced penicillin susceptibility (PRGBS) by commercially available methicillin-resistant-Staphylococcus aureus (MRSA)-selective agar
Diagnostic Microbiology and Infectious Disease in press ¶ Equally contribution

 

  R. Taniguchi, K. Kimura*, A. Miyazaki, H. Banno, W. Jin, K. Yamada, J. Wachino, Y. Arakawa (2016)

High rate of slowly-killed-by-ampicillin phenotype among group B streptococci with reduced penicillin susceptibility (PRGBS)

The Journal of Antimicrobial Chemotherapy in press.

*corresponding author

M. Inaba, N. Matsuda, H. Banno, W. Jin, J. Wachino, K. Yamada, K. Kimura, Y. Arakawa (2016)

In vitro reduction of antibacterial activity of tigecycline against multidrug-resistant Acinetobacter baumannii with host stress hormone norepinephrine

International Journal of Antimicrobial Agents in press.

  J. Wachino, Y. Yamaguchi, S. Mori, W. Jin, K. Kimura, H. Kurosaki, Y. Arakawa (2016)

"Structural insights into recognition of hydrolyzed carbapenems and inhibitors by subclass B3 metallo-β-lactamase SMB-1"

Antimicrobial Agents and Chemotherapy 60(7): 4274-82

2015

Fujita A,Kimura K,Yokoyama S,Jin W,Wachino J,Yamada K, Suematsu H,Yamagishi Y,Mikamo H,Arakawa Y.
Characterization of piperacillin/tazobactam-resistant Klebsiella oxytoca recovered from a nosocomial outbreak.
PLOS ONE,in press.

Seki T, Kimura K, Reid M.E, Miyazaki A, Banno H, Jin W, Wachino J, Yamada K, Arakawa Y.
High isolation rate of multi-drug resistant group B streptococci with reduced penicillin susceptibility in Japan.
The Journal of Antimicrobial Chemotherapy.in press.

Kasama T, Ikami M, Jin W, Yamada K, Kaji N, Atsumi Y. Mizutani M, Okamoto A, Namikawa T, Ohta M, Tokeshi M, Baba Y.
Rapid, highly sensitive, and simultaneous detection of staphylococcal enterotoxins in milk by using immuno-pillar devices.
Analytical Methods.in press,2015.

Kamiya C, Kimura K, Doyama Y, Miyazaki A, Morimoto M, Banno, H, Nagano N, Jin W, Wachino J,Yamada K, Arakawa Y.
Ceftibuten-Containing Agar Plate for Detecting Group B Streptococci with Reduced Penicillin Susceptibility (PRGBS).
Diagnostic Microbiology and Infectious Disease. 82(4):269-273,2015.

Kimura K, Nagano N, Arakawa Y.
Classification of Group B Streptococci with Reduced β-Lactam Susceptibility (GBS-RBS) Based on the Amino Acid Substitutions in Penicillin-binding Proteins (PBPs).
The Journal of Antimicrobial Chemotherapy.70(6):1601-1603,2015.

Jin W, Wachino J, Kimura K, Yamada K, Arakawa Y.
New plasmid-mediated aminoglycoside 6'-N-acetyltransferase, AAC(6')-Ian, and extended-spectrum β-lactamase, TLA-3, from Serratia marcescens clinical isolate.
J Antimicrob Chemother.70(5):1331-1337,2015.

Yamada R, Kimura K, Nagano N, Nagano Y, Suzuki S, Jin W, Wachino J, Yamada K, Shibayama K, Arakawa Y.
Comparative Analysis of Penicillin-Susceptible and Non-Susceptible Isolates in Group B Streptococci by Multilocus Sequence Typing.
Japanese Journal of Infectious Diseases.68(4):326-329,2015.

Yamaguchi Y, Matsueda S, Matsunaga K, Takashio N, Toma-Fukai S, Yamagata Y, Shibata N, Wachino J, Shibayama K, Arakawa Y, Kurosaki H.
Crystal structure of IMP-2 metallo-β-lactamase from Acinetobacter spp.: Comparison of active-Site loop structures between IMP-1 and IMP-2.
Biol Pharm Bull.38(1):96-101,2015.

Suzuki T, Kimura K, Suzuki H, Banno H, Jin W, Wachino J, Yamada K, Arakawa Y.
Have group A streptococci with reduced penicillin susceptibility emerged?
J Antimicrob Chemother.70(4):1258-1259,2015.

Nagasaka Y, Kimura K, Yamada K, Wachino J, Jin W, Notake S, Yanagisawa H, Arakawa Y.
Genetic profiles of fluoroquinolone-nonsusceptible Klebsiella pneumoniae among cephalosporin-resistant K. pneumoniae.
Microb Drug Resist.(2):224-233.2015.

Ito R, Shindo Y, Kobayashi D, Ando M, Jin W, Wachino J, Yamada K, Kimura K, Yagi T, Hasegawa Y, Arakawa Y.
Molecular Epidemiological Characteristics of Klebsiella pneumoniae Associated with Bacteremia among Patients with Pneumonia.
Journal Clinical Microbiology.53(3):879-886,2015.

Umene YD, Wong LK, Satoh T,Yamane K,Matsui M,Riley LW, Arakawa Y,Suzuki S.
Molecular epidemiological characterization of uropathogenic Escherichia coli from an outpatient urology clinic in rural Japan.
J Clin Microbiol.53(2):681-683,2015.

Asai K,Yamada K,Yagi T,Baba H,Kawamura I,Ohta M.
Effect of incubation atmosphere on the production and composition of staphylococcal biofilms  Journal of Infection and Chemotherapy.
21(1):55–61,2015.

2014

Nagasawa M, Kaku M, Kamachi K, Shibayama K, Arakawa Y, Yamaguchi K, Ishii Y.
Loop-mediated isothermal amplification assay for 16S rRNA methylase genes in Gram-negative bacteria.
J Infect Chemother. 20:635-638,2014.

Mori S, Kim H,  Rimbara E, Arakawa Y, Shibayama K.
Roles of Ala-149 in the catalytic activity of diadenosine tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv.
Biosci Biotechnol Biochem.28:1-3,2014.

Wachino J, Kimura K, Yamada K, Jin W, Arakawa Y.
Evaluation of disk potentiation test using KB disks containing high dosage fosfomycin and glucose-6-phosphate to detect the production of glutathione S-transferase responsible for fosfomycin resistance.
J Clin Microbiol.(10):3827-3828,2014.

Kitanaka H, Sasano M, Yokoyama S, Suzuki M, Jin W, Inayoshi M, Hori M, Wachino J, Kimura K, Yamada K, Arakawa Y.
Carbapenem-resistant Acinetobacter soli as a newly emerging invasive pathogen.
Emerg. Infect. Dis. 20(9):1574-1576, 2014.

Nakamura G, Wachino J, Sato N, Kimura K, Yamada K,  Jin W, Shibayama K, Yagi T , Kawamura K, Arakawa Y.
Practical Agar-Based Disk Potentiation Test to Detect Fosfomycin−Non-Susceptible Escherichia coli Clinical Isolates Producing Glutathione S-Transferases.
J Clin Microbiol. 52(9):3175-3179,2014.

Nagano N, Nagano Y, Toyama M, Kimura K, Shibayama K, Arakawa Y. 
Penicillin-susceptible group B streptococcal clinical isolates with reduced cephalosporin susceptibility.
J Clin Microbial. 52(9):3406-3410,2014.

 

Suzuki M, Hosoba E, Matsui M, Arakawa Y.
New PCR-Based Genotyping Method for Easy, Rapid and Reliable Identification of Acinetobacter baumannii International Epidemic Clones without Performing MLST.
J. Clin. Microbiol.58(8):2925-2932,2014.

Kitanaka H, Wachino J, Jin W, Yokoyama S, Sasano M, Hori M, Yamada K, Kimura K, Arakawa Y.
Novel integron-mediated fosfomycin-resistance genetic determinant, fosK.
Antimicrob Agents Chemother. 58(8):4978-4979,2014.

Okamoto A, Lee H, Yabutani M, Yamada K, Ohta M.
Draft genome sequence of a Legionella pneumophila serogroup 4 strain causing legionellosis.
Genome Announc.2(3):602-614,2014.

Banno H, Kimura K, Tanaka Y, Kitanaka H, Jin W, Wachino J, Yamada K, Shibayama K, Arakawa Y.
Characterization of Multi-drug-Resistant Group B Streptococci with Reduced Penicillin Susceptibility (PRGBS) Forming Small Non-β-hemolytic Colonies on Sheep Blood Agar Plates.
J Clin Microbiol. 52(6):2169-2171.2014.

Matsui M, Suzuki S, Yamane K, Suzuki M, Konda T, Arakawa Y, Shibayama K.
Distribution of carbapenem resistance determinants among epidemic and non-epidemic types of Acinetobacter species in Japan.
J Med Microbiol. 63(pt 6):870-877, 2014.

Bart MJ,  Harris SR,  Advani A,  Arakawa Y,  Bottero D,  Bouchez V,  Cassiday PK,  Chiang CS,  Dalby T,  Fry NK,  Gaillard ME,  van Gent M,  Guiso N,  Hallander HO,  Harvill ET,  He Q,  van der Heide HG,  Heuvelman K,  Hozbor DF,  Kamachi K,  Karataev GI,  Lan R,  Lutyłska A,  Maharjan RP,  Mertsola J,  Miyamura T,  Octavia S,  Preston A,  Quail MA, Sintchenko V,  Stefanelli P,  Tondella ML,  Tsang RS,  Xu Y,  Yao SM,  Zhang S,  Parkhill J,  Mooi FR.
Global Population Structure and Evolution of Bordetella pertussis and Their Relationship with Vaccination.
MBio. 5(2):e01074-14,2014. 

Kawamura K, Goto K, Nakane K, Arakawa Y.
Molecular epidemiology of extended-spectrum β-lactamases and Escherichia coli isolated from retail foods including chicken meat in Japan.
Foodborne Pathog Dis.11(2):104-110,2014.

2013

Matsui M,  Suzuki S,  Suzuki M,  Arakawa Y,  Shibayama K.
Rapid discrimination of Acinetobacter baumannii international clone II lineage by pyrosequencing SNP analyses of bla(OXA-51-like) genes.
J Microbiol Methods.94(2):121-124,2013.

Ato M, Takahashi Y,  Fujii H,  Hashimoto S,  Kaji T,  Itamura S,  Horiuchi Y,  Arakawa Y,  Tashiro M,Takemori T.
Influenza A whole virion vaccine induces a rapid reduction of peripheral blood leukocytes via interferon-α-dependent apoptosis.
Vaccine.31(17):2184-2190,2013.

Kimura K,  Yanagisawa H,   Wachino J,  Shibayama K,  Arakawa Y.
Rapid and reliable loop-mediated isothermal amplification method for detecting Streptococcus agalactiae.
Japanese Journal of Infectious Diseases.66(6):546-548, 2013.

Nagano N,  Endoh Y,  Nagano Y,  Toyama M,  Matsui M,  Shibayama K,  Arakawa Y.
First Report of OXA-48 Carbapenemase-Producing Klebsiella pneumoniae and Escherichia coli in Japan from a Patient Returned from Southeast Asia.
Jpn J Infect Dis.66:79-81,2013.

Matsuoka M, Sasaki T, Seki N, Kobayashi M, Sawabe K, Sasaki Y, Shibayama K, Sasaki T, Arakawa Y.
Hemin-binding proteins as potent marker for serological diagnosis of infections with Bartonella quintana.
Clin Vaccine Immunol.20(4):620-626,2013.

Wachino J,  Yamaguchi Y,  Mori S,  Kurosaki H,  Arakawa Y,  Shibayama K.
Structural insights into the subclass B3 metallo-β-lactamase SMB-1 and the mode of inhibition by the common metallo-β-lactamase inhibitor mercaptoacetate.
Antimicrob Agents Chemother.57:101-109,2013.

Jin W,  Yamada K,  Ikami M,  Kaji N,  Tokeshi M,  Atsumi Y,  Mizutani M,  Murai A,  Okamoto A,  Namikawa T,  Baba Y,  Ohta M.
Application of IgY to sandwich enzyme-linked immunosorbent assays, lateral flow devices, and immunopillar chips for detecting staphylococcal enterotoxins in milk and dairy products.
Journal of Microbiological Methods.92:323–331,2013.

Kimura K, Wachino J, Kurokawa H, Matsui M, Suzuki S, Yamane K, Nagano N, Shibayama K, Arakawa Y.
High cephalosporin resistance due to amino acid substitutions in PBP1A and PBP2X in a clinical isolate of group B streptococcus.
The Journal of Antimicrobial Chemotherapy.68(7):1533-1536,2013.

Kimura K,  Nishiyama Y,  Shimizu S,  Wachino J,  Matsui M,  Suzuki S,  Yamane K,  Shibayama K,  Arakawa Y.
Screening for Group B Streptococci with Reduced Penicillin. Susceptibility in Clinical Isolates Obtained between 1977 and 2005.
Jan J Infect DIs.66(3):222-225,2013.

 

Kimura K,  Matsubara K,  Yamamoto G,  Shibayama K,  Arakawa Y.
Active Screening of Group B Streptococci with Reduced Penicillin Susceptibility and Altered Serotype Distribution, Isolated from Pregnant Women in Kobe, Japan.Japanese Journal of Infectious Diseases.66(2):158-160, 2013.

Kimura K,  Nagano N,  Nagano Y,  Wachino J,  Shibayama K,  Arakawa Y.
Ability of the VITEK® 2 System to Detect Group B Streptococci with Reduced Penicillin susceptibility.
The Journal of Antimicrobial Chemotherapy.68(6):1442-1444,2013.

Yamada K, Jin W, Ohkura T, Murai A, Hayakawa R, Kinoshita K, Mizutani M, Okamoto A, Namikawa T, Ohta M.
Detection of Methicillin-Resistant Staphylococcus aureus Using Specific Anti-PBP2a Chicken IgY Antibody.
Japanese Journal of Infectious Diseases.66(2):103-108, 2013.

Kimura K, Nagano N, Nagano Y,Suzuki S, Wachino J, Shibayama K, Arakawa Y.
High Frequency of Fluoroquinolone- and Macrolide-resistant Streptococci among Clinically Isolated Group B Streptococci with Reduced Penicillin Susceptibility.
The Journal of Antimicrobial Chemotherapy.68(3):539-542,2013.

2012

Nomura T, Tanimoto K, Shibayama K, Arakawa Y, Fujimoto S, Ike Y, Tomita H.
Identification of VanN-type vancomycin resistance in an Enterococcus faecium isolate from chicken meat in Japan.
Antimicrob Agents Chemother.56:6389-6392,2012.

Wachino J,  Arakawa Y.
Exogenously acquired 16S rRNA methyltransferases found in aminoglycoside-resistant pathogenic Gram-negative bacteria:an update.
Drug Resist Updat.15:133-148,2012.

Wachino J, Yamaguchi Y, Mori S, Yamagata Y, Arakawa Y, Shibayama K.
Crystallization and preliminary X-ray analysis of the subclass B3 metallo-β-lactamase SMB-1 that confers carbapenem resistance.
Acta Crystallogr Sect F Struct Biol Cryst Commun.68:343-346,2012.

Otsuka N, Han HJ, Toyoizumi-Ajisaka H, Nakamura Y, Arakawa Y, Shibayama K, Kamachi K,
Prevalence and genetic characterization of pertactin-deficient Bordetella pertussis in Japan.
PLoS One.7(2):e31985,2012.

Nagano N, Nagano Y, Toyama M, Kimura K, Tamura T, Shibayama K, Arakawa Y.
Nosocomial spread of multidrug-resistant group B streptococci with reduced penicillin susceptibility belonging to clonal complex 1.
The Journal of Antimicrobial Chemotherapy,67:849-856,2012.

Lee SY, Park YJ, Yu JK, Jung S, Kim Y, Jeong SH, Arakawa Y,
Prevalence of acquired fosfomycin resistance among extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae clinical isolates in Korea and IS26-composite transposon surrounding fosA3.
The Journal of Antimicrobial Chemotherapy,67:2843-2847,2012.

Takeno A,  Okamoto A, Torii K,  Oshima K,  Hirakawa H,  Toh H,  Agata N,  Yamada K,  Ogasawara N,  Hayashi T,  Shimizu T,   Kuhara S,  Hattori M, and Ohta M,
Complete Genome Sequence of Bacillus cereus NC7401, Which Produces High Levels of the Emetic Toxin Cereulide.
 J Bacteriol 194(17):4767-4768, 2012.

Nagano N, Nagano Y, Toyama M, Kimura K, Tamura T, Shibayama K,  Arakawa Y.
Nosocomial spread of multidrug-resistant group B streptococci with reduced penicillin susceptibility belonging to clonal complex 1.
The Journal of Antimicrobial Chemotherapy.67:849-856, 2012.

2011

Sekizuka T, Matsui M, Yamane K, Takeuchi F, Ohnishi M, Hishinuma A, Arakawa Y, Kuroda M,
Complete sequencing of the bla(NDM-1)-positive IncA/C plasmid from Escherichia coli ST38 isolate suggests a possible origin from plant pathogens.
PLoS One.6(9):e25334,2011.

Mori S,  Shibayama K,  Wachino J,  Arakawa Y.
Structural insights into the novel diadenosine 5',5‴-P¹,P⁴-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv.
J Mol Biol.410:93-104,2011.

Han HJ, Kuwae A, Abe A, Arakawa Y, Kamachi K,
Differential expression of type III effector BteA protein due to IS481 insertion in Bordetella pertussis.
PLoS One.6(3):e17797,2011.

Shibayama K,  Takeuchi H,  Wachino J,  Mori S,  Arakawa Y.
Biochemical and pathophysiological characterization of Helicobacter pylori asparaginase.
Microbiol Immunol.55:408-417,2011.

Okamoto A, Yamada K.
Proteome driven re-evaluation and functional annotation of the Streptococcus pyogenes SF370 genome. 
BMC Microbiology.11:249,2011.

Okamoto A, Hasegawa T, Yamada K, and Ohta M. Application of both high-performance liquid chromatography combined with tandem mass spectrometry shotgun and 2-D polyacrylamide gel electrophoresis for streptococcal exoproteins gave reliable proteomic data.
Microbiol Immunol.55(2):84-94,2011.

 

Tomita Y, Ohta M, Yamada K, Okamoto A, Yagi T, and Hasegawa Y.
A new microarray system to detect Streptococcus pneumoniae serotypes.
J Biomed Biotech.352736,2011.

 

Tomita Y, Okamoto A, Yamada K, Yagi T, Hasegawa Y, and Ohta M.
Capsular-type prediction by phylogenetic tree of glycosyltransferase gene polymorphism in Streptococcus pneumoniae.
Open Access Bioinformatics.3:67-73,2011.

 

Kimura K, Nagano N, Nagano Y, Wachino J, Suzuki S, Shibayama K, Arakawa Y.
Predominance of sequence type 1group with serotype VI among group B streptococci with reduced penicillin susceptibility identified in Japan.
The Journal of Antimicrobial Chemotherapy.66:2460-2464, 2011.

 

Wachino J, Yoshida H, Yamane K,  Suzuki S, Matsui M, Yamagishi T, Tsutsui A,  Konda T, Shibayama K, Arakawa Y.
SMB-1, a novel subclass B3metallo-β-lactamase, associated with ISCR1 and a class 1 integron,from a carbapenem-resistant Serratia marcescens clinical isolate.
Antimicrob Agents Chemother. 55:5143-5149,2011.

 

Wachino J, Yamane K, Arakawa Y.
Practical disk-based method for detection of Escherichia coli clinical isolates producing the fluoroquinolon e-modifying enzyme AAC(6')-Ib-cr.
J Clin Microbiol.49:2378-2379,2011.

 

Tsutsui A , Suzuki S , Yamane K , Matsui M , Konda T, Marui E, Takahashi K , Arakawa Y.
Genotypes and infection sites in an outbreak of multidrug-resistant Pseudomonas aeruginosa. 
Journal of HospitalInfection.78:317-322,2011.

 

Yamaguchi Y,  Imamura K,  Sasao A,  Murakami E,  Arakawa Y,  Kurosaki H.
Metal Preference of Zn(II)and Co(II) for DinuclearMetal Binding Site of IMP-1Metallo-β-lactamase and spectroscopic.properties of Co(II)-substituted IMP-1 with Mercaptoacetic acid.
Med. Chem. Commun.2:720-725,2011.

 

 Yamaguchi Y, Ding S, Murakami E, Imamura K, Fuchigami S, Hashiguchi R, Yutani K, Mori H, Suzuki S, Arakawa Y, Kurosaki H .
A Demetallation Method for IMP-1Metallo-β-lactamase That Has Restored Enzymatic Activity Upon Addition of Metal Ion(s).
ChemBioChem.12:1979-1983,2011.

2010

Wachino J, Shibayama K, Kimura K, Yamane K, Suzuki S, Arakawa Y.
RmtC introduces G1405 methylation in 16S rRNA and confers high-level aminoglycoside resistance on Gram-positive microorganisms.
 FEMS Microbiol Lett.311:56-60,2010.


 

Minauchi K, Takahashi S, Sakai T, Kondo M, Shibayama K, Arakawa Y, Mukai M.
The nosocomial transmission of Helicobacter cinaedi infections in immunocompromised patients.
Intern Med.49:1733-1739,2010.

Wachino J, Shibayama K, Suzuki S, Yamane K, Mori S, Arakawa Y.
Profile of Expression of Helicobacter pylori gamma-glutamyltranspeptidase.
Helicobacter.15:184-192,2010. 

Nakamura Y, Kamachi K, Toyoizumi-Ajisaka H, Otsuka N, Saito R, Tsuruoka J, Katsuta T, Nakajima N, Okada K, Kato T, Arakawa Y.
Marked difference between adults and children in Bordetella pertussis DNA load in nasopharyngeal swabs.
Clin Microbiol Infect.17:365-370,2010.

Iwaki M, Komiya T, Yamamoto A, Ishiwa A, Nagata N, Arakawa Y, Takahashi M.
Genome organization and pathogenicity of Corynebacterium diphtheriae C7(-) and PW8 strains.
Infect Immun.78:3791-3800,2010.

Kawamura-Sato K, Wachino J, Kondo T, Ito H, Arakawa Y.
Correlation between disinfectant-reduced susceptibility and multidrug resistance among clinically isolated Acinetobacter species.
J Antimicrob Chemother.65:1975-8,2010.

 

Kuroda M, Katano H, Nakajima N, Tobiume M, Ainai A, Sekizuka T, Hasegawa H, Tashiro M, Sasaki Y, Arakawa Y, Hata S, Watanabe M, Sata T.
Characterization of quasispecies of pandemic 2009 influenza A virus (A/H1N1/2009) by de novo sequencing using a next-generation DNA sequencer.
PLoS One.5(4):e10256,2010.

 

Wachino J, Yamane K, Suzuki S, Kimura K, Arakawa Y.
Prevalence of fosfomycin resistance among CTX-M-producing Escherichia coli clinical isolates in Japan and identification of novel plasmid-mediated fosfomycin-modifying enzymes.
Antimicrob Agents Chemother.54(7):3061-3064, 2010.

 

Yamaguchi Y, Takashio N, Wachino J, Yamagata Y, Arakawa Y, Matsuda K, Kurosaki H.
Structure of metallo-beta-lactamase IND-7 from a Chryseobacterium indologenes clinical isolate at 1.65 Å resolution.
J Biochem.147(6):905-915,2010.


 

Mori S, Shibayama K, Wachino J, Arakawa Y.
Crystallization and preliminary X-ray analysis of the diadenosine 5',5'''-P1, P4-tetraphosphate phosphorylase from Mycobacterium tuberculosis H37Rv.
Acta Crystallogr Sect F Struct Biol Cryst Commun.66:279-281,2010.

 

Kato H, Kato H, Ito Y, Akahane T, Izumida S, Yokoyama T, Kaji C, Arakawa Y.
Typing of Clostridium difficile isolates endemic in Japan by sequencing of slpA and its application to direct typing.
J Med Microbiol.59:556-562,2010.

 

Kamochi K, Fukuda T, Han HJ, Toyoizumi-Ajisaka H, Mochida K, Konda T, Horiuchi Y, Arakawa Y.
 DPT Technical Committee of the Association of Biological Manufacturers of Japan. Genetic verification of Bordetella pertussis seed strains used for production of Japanese acellular pertussis vaccines.
Biologicals.38:290-293,2010.

 

Shiota M, Saitou K, Mizumoto H, Matsusaka M, Agata N, Nakayama M, Kage M,Tatsumi S,Okamoto A, Yamaguchi S, Ohta M, Hata D.
Rapid detoxification of Cereulide in Bacillus cereus food poisoning.
Pediatrics.125:951-955,2010.

 

Hasegawa T, Okamoto A, Kamimura T, Tatsuno I, Hashikawa S, Yabutani M, Matsumoto M, Yamada K, Isaka M, Minami M, Ohta M.
Detection of invasive protein profile of Streptococcus pyogenes M1 isolates from pharyngitis patients.
APMIS.118(3):167-178,2010.

 

Yamada K, Inuzuka K, Tatsumi N, Sanzen I, Ohkura T, Okamoto A, Hasegawa T, Ohta M.
Evaluation of selection media for the detection of borderline MRSA.
J Infect Chemother.16:19-24,2010.

 


Kawamura-Sato K, Yoshida R, Shibayama K, Ohta M.
Virulence genes, quinolone and fluoroquinolone resistance, and phylogenetic background of uropathogenic Escherichia coli strains isolated in Japan.
Jpn J Infect Dis.63:113-115,2010.

 

Hasegawa T, Minami M, Okamoto A, Tatsuno I, Isaka M, Ohta M.
Characterization of a virulence-associated and cell-wall-located DNase of Streptococcus pyogenes.
Microbiol.156:184-190,2010.

 

 

Minami M, Kamimura T, Isaka M, Tatsuno I, Ohta M, Hasegawa T.
Clindamycin-induced CovS-mediated regulation of the production of virulent exoproteins streptolysin O, NAD glycohydrolase, and streptokinase in Streptococcus pyogenes.
Antimicrob Agents Chemother.54:98-102,2010.

2009

Kimura K, Wachino J, Kurokawa H, Suzuki S, Yamane K, Shibata N, Arakawa Y.
Practical disk diffusion test for detecting group B Streptococcus with reduced penicillin susceptibility.
J Clin Microbiol.47:4154-4157,2009.

 Nagano N, Kimura K, Nagano Y, Yakumaru H, ArakawaY.                                                         

Molecular characterization of group B streptococci with reduced penicillin susceptibility recurrently isolated from a sacral decubitus ulcer.
J Antimicrob Chemother.64:1326-1328,2009.

 

Mori S, Shibayama K, Wachino J, Arakawa Y.
Purification and molecular characterization of a novel diadenosine 5', 5'''-P(1), P(4)-tetraphosphate phosphorylase from Mycobacterium tuberculosis H27Rv.
Protein Expr Purif.69:99-105,2009.
 

Honma Y, Yoshii Y, Watanabe Y, Aoki N, Komiya T, Iwaki M, Arai H, Arakawa Y, Takahashi M, Kimura H.
A case of afebrile pneumonia caused by non-toxigenic Corynebacterium diphtheriae.
Jpn J Infect Dis.62:327-329,2009. 

 

Yamaguchi Y, Sato G, Yamagata Y, Doi Y, Wachino J, Arakawa Y, Matsuda K, Kurosaki H.
Structure of AmpC beta-lactamase (AmpCD) from an Escherichia coli clinical isolate with a tripeptide deletion (Gly286-Ser287-Asp288) in the H10 helix.
Acta Crystallogr Sect F Struct Biol Cryst Commun.65:540-543,2009.
 

Park YJ, Yu JK, Kim SI, Lee K, Arakawa Y.
Accumulation of plasmid-mediated fluoroquinolone resistance genes, qepA and qnrS1, in Enterobacter aerogenes co-producing RmtB and class A beta-lactamase LAP-1.
Ann Clin Lab Sci.39:55-59,2009.


Piao Z, Shibayaka K, Mori S, Wachino J, Arakawa Y.
A novel insertion sequence, IS1642, of Mycobacterium  avium, which forms long direct repeats of variable length.
FEMS Microbiol Lett.291:216-221,2009.


Suzuki S, Shibata N, Yamane K, Wachino J, Ito K, Arakawa Y.
Change in the prevalence of extended-spectrum-beta-lactamase-producing Escherichia coli in Japan by clonal spread.
J Antimicrob Chemother.63:72-79,2009.

 

Nagano Y, Nagano N, Wachino J, Ishikawa K, Arakawa Y.
Novel chimeric beta-lactamase CTX-M-64, a hybrid of CTX-M-15-like and CTX-M-14-beta-lactamases, found in a Shigella sonnei strain resistant to various oxyimino-cephalosporins, including ceftazidime.
Antimicrob Agents Chemother.53:69-74,2009.

 

 
Minami M, Ando T, Okamoto A, Sasaki N, Ohkura T, Torii K, Hasegawa T, Ohta M, Goto H.
Seroprevalence of Fusobacterium varium in ulcerative colitis patients in Japan.
Immunol Med Microbiol.56(1):67-72,2009. 

 

Ishihara Y, Hyodo M, Hayakawa Y, Kamegaya T, Yamada K, Okamoto A, Hasegawa T,  Ohta M.
Effect of cyclic bis(3’-5’) diguanylic acid and its analogs on bacterial biofilm formation.
FEMS Microbiol Lett.301(2):193-200,2009.

 


 Ohkura T, Yamada K, Okamoto A, Baba H, Ike Y, Arakawa Y, Hasegawa T, Ohta M.
Nationwide epidemiological study revealed the dissemination of methicillin-resistant Staphylococcus aureus carrying a specific set of virulence-associated genes in Japanese hospitals.
J Med Microbiol.58:1329-1336,2009.

 


Baba H, Iinuma Y, Imaizumi K, Hasegawa Y, Hasegawa T, Ohta M, Paterson DL.
Transmission of Bacterial Infections to Healthcare Workers during Intubation and Respiratory Care of Patients with Severe Pneumonia.
Infect Control Hosp Epidemiol.30(10):1019-1021,2009.

 

Nada T, Yagi T, Ohkura T, Morishita Y, Baba H, Ohta M, Suzuki M.
Usefulness of phage open-reading frame typing method in and epidemiological study of an outbreak of methicillin-resistant staphylococcus aureus infections.
Jpn J Infect Dis.62(5):386-389,2009. 

 

 Minami M, Ohmori D, Tatsuno I, Isaka M, Kawamura Y, Ohta M, Hasegawa T.
The streptococcal inhibitory of complement(SIC) protects Streptococcus pyogenes from bacteriocin-like inhibitory substance(BLIS) from Streptococcus salivarius.
FEMS Microbiol Lett.298(1):67-73,2009.

 

Yabutani M, Agata N, Ohta M.
 A new rapid and sensitive detection method for cereulide-producing Bacillus cereus using a cycleave real-time PCR.
 Lett Appl Microbiol.48(6):698-704,2009.

 

Nagao M, Okamoto A, Yamada K, Hasegawa T, Hasegawa Y, Ohta M.
Variations in amount of TSST-1 produced by clinical methicillin resistant Staphylococcus aureus (MRSA) isolates and allelic variation in accessory gene regulator (agr) locus.
BMC Microbiology.9:52,2009. 

 

 Kondo Y, Tokuda N, Fan X, Yamashita T, Honke K, Takematsu H, Togayachi A, Ohta M, Kotzusumi Y, Narimatsu H, Tajima O, Furukaw K, Furukawa K.
Glycosphingolipids are not pivotal receptors for Subtilase cytotoxin in vivo: sensitivity analysis with glycosylation-defective mutant mice.
Biochem Biophys Res Commun.378(2):179-181,2009. 

 

Ishikawa H, Takeda K, Okamoto A, Matsuo S, Isobe K. Induction of autoimmunity in a bleomycin-induced murine model of experimental systemic sclerosis: An important role for CD4(+) T Cells.
J Invest Dermatol.129(7):1688-1695,2009.